Description: The human sICAM-3 Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of Intercellular Adhesion Molecule-3. The human sICAM-3 Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.
ICAM-3 is a heavily glycosylated, integral membrane protein with five immunoglobulin-like domains. It shares high homology to ICAM-1 and ICAM-2 in the extracellular region. In contrast to ICAM-1 and ICAM-2, ICAM-3 is absent on endothelia. ICAM-3 is expressed on resting lymphocytes, monocytes and neutrophils, representing the major LFA-1 ligand on these cells. The finding that adhesion of resting T lymphocytes to LFA-1 occurs primarily via ICAM-3 combined with the fact that ICAM-3 is much better expressed than other LFA-1 ligands on monocytes and resting lymphocytes implies an important role for ICAM-3 in the initiation of immune responses.
The initial interaction of ICAM-3 with LFA-1 might increase LFA-1-mediated cell binding to ICAM-1.
Furthermore, ICAM-3 expression has been shown for dendritic epidermal Langerhans cells, whereas it is absent on other dendritic cells from different lymphoid organs. Thus potential function of ICAM-3 at the initiation phase of LC-leukocyte interactions taking place during skin localized immune reactions can be postulated.
Components
Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human sICAM-3, Sample Diluent and HRP-Conjugate (anti-sICAM-3 monoclonal (murine) antibody), lyophilized
Aluminium pouch(es) with a human sICAM-3 Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Sample Diluent (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers
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