Description: The human IFN-alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IFN-alpha. The human IFN-alpha ELISA is for in vitro diagnostic use. Not for use in therapeutic procedures.
The interferons represent proteins with antiviral activity secreted from cells in response to a variety of stimuli. In mammals, class I interferon (IFN-alpha) genes form a superfamily consisting of three gene families, the alpha interferon (IFN-alpha), the beta interferon (IFN-beta) and the interferon omega (IFN-omega) genes. In humans the IFN-alpha family comprises more than 20 genes and pseudogenes giving rise to 15 different functional gene products. The various species of human IFN-alpha are closely related in amino acid sequences with homologies in the range of 80 to 100 %.
During the acute phase of a viral infection IFN-alpha levels are significantly elevated in the majority of patients. IFN-alpha levels fall significantly during the period of convalescence at the time viral infection is indicated by seroconversion tests.
Increased levels of IFN-alpha were found in the majority of patients suffering from inflammatory arthropathies like juvenile polyarthritis, rheumatoid arthritis, ancylosing spondylitis, polychondritis, psoriatic arthritis, polymyalgia rheumatica and sclerodermia. Elevated IFN-alpha levels have also been shown for other autoimmune disorders like systemic lupus erythematosus and systemic vasculitis.
Serum IFN-alpha levels can help to distinguish between children with non-specific abdominal pain or mesenteric adenitis, and those with acute appendicitis.
Intrapleurally administered interferon causes measurable serum concentrations which correlated with the degeneration of malignant cells.
The IFN-alpha product is manufactured and sold in the United States under license from Pestka Biomedical Laboratories, Inc. (d/b/a PBL InterferonSource) solely for research use in the form in which it is originally manufactured, packaged and sold. Any modification, repackaging, or alteration of the product, and any use for diagnostic, therapeutic or clinical purposes is strictly prohibited.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IFN-alpha
HRP-Conjugate anti-human IFN-alpha monoclonal antibody
Human IFN-alpha Standard, 500 pg/mL
Control high, lyophilized
Control low, lyophilized
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films
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