HU IL-10 HS COATED ELISA

• 产品编号：BMS215HS      品牌：invitrogen       原厂货号：BMS215HS
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

描述：

Description: This High Sensitivity ELISAs enables the analysis of Interleukin 10 in low concentrations from serum, plasma, or cell culture samples.

eBioscience High Sensitivity ELISA Kits offer accurate quantification below conventional ELISA limits. This is made possible by ther inherent design by amplifying the signal generated by the enzyme horseradish peroxidase (HRP) and the colorimetric-detectable substrate tetramethylbenzidin (TMB) based on Tyramide Signal Amplification (TSA) technology*.

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones.
Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four a-helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked, glycosylation site, it lacks detectable carbohydrates. Recombinant protein expressed in E. coli thus retains all known biological activities. The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.

*High Sensitivity ELISAs are based on a patent from PerkinElmer LAS, patent number 5,731,158; 5,583,001; 5,196,306.

原厂资料：