Description: The human sTNF-R (80 kDa) ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sTNF-R (80 kDa). The human sTNF-R (80 kDa) ELISA is for in vitro diagnostic use. Not for use in therapeutic procedures.
Tumor Necrosis Factor (TNF) was originally discovered in sera of animals and was found to cause hemorrhagic necrosis of some transplantable mouse and human tumors and to exhibit primarily cytotoxic activities against tumor but not normal cells in vitro. The TNF family consists of two proteins designated TNF-alpha, also called cachectin, and TNF-beta, also called lymphotoxin, which are pleiotropic cytokines that can mediate a wide variety of biological effects.
Both TNF-alpha and TNF-beta have been shown to interact with a cell through specific high affinity receptors with a few hundred up to more than 20,000 copies per cell. TNF-receptors have been demonstrated on a wide variety of human somatic cells including fibroblasts, endothelial cells, adipocytes, liver membranes, granulocytes and several tumor cell lines.
The predicted amino acid sequences of the extracellular regions of the two TNF-R reveal significant similarities. They share 28 % identity on their extracellular domain, but their intracellular parts are totally different from each other.
The present assay provides a simple, rapid and highly sensitive method for the determination of soluble TNF-R (80 kDa) levels in body fluids or cell culture supernatants. This assay will help to clarify the possible diagnostic and prognostic value of circulating sTNF-R (80 kDa) in various neoplastic and inflammatory diseases.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sTNF-R (80 kDa)
HRP-Conjugate anti-human sTNF-R (80 kDa) monoclonal antibody
Human sTNF-R (80 kDa) Standard, 10 ng/ml
Control, lyophilized
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films
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