human TNF-R (60kDa) Platinum ELISA

• 产品编号：BMS203CE      品牌：eBioscience       原厂货号：BMS203CE
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

描述：

Description: The human sTNF-R (60 kDa) ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sTNF-R (60 kDa). The human sTNF-R (60 kDa) ELISA is for in vitro diagnostic use. Not for use in therapeutic procedures.

TNF-receptors have been demonstrated on a wide variety of human somatic cells including fibroblasts, endothelial cells, adipocytes, liver membranes, granulocytes and several tumor cell lines. Based on gel filtration experiments the receptor appears to be a complex of different proteins with a molecular weight of 350 kDa. In a variety of cell lines two different types of TNF receptors with 75-80 and 55-60 kDa respectively have been identified. The cDNAs encoding the two different TNF receptors have been cloned. The exact mechanism of signal transduction after binding of TNF to the receptor is still unclear. The extracellular fragment of the 60 kDa TNF receptor, with a molecular mass of about 30 kDa was purified, partially sequenced, and the respective cDNA was cloned. This TNF binding protein is liberated from the intact molecule by proteolytic cleavage and comprises most of the extracellular portion of the receptor, including all three N-glycosylation sites.

The present assay provides a simple, rapid, and highly sensitive method for the determination of soluble TNF-R (60 kDa) levels in body fluids or cell culture supernatants. This assay will help to clarify the possible diagnostic and prognostic value of circulating sTNF-R (60 kDa) in various neoplastic and inflammatory diseases.

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