Description: The human IL-17F ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-17F. The human IL-17F ELISA is for research use only. Not for diagnostic or therapeutic procedures.
IL-17F and IL-17A are closely related members of the IL-17 cytokine family, sharing about 50% amino acid identity. Expression of IL-17F and IL-17A has been detected in activated human peripheral blood lymphocytes and has also been observed in tissue samples from various autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory bowel disease, and asthma.
The crystal structure of IL-17F has been solved and shows that the protein forms a disulfide-linked dimeric glycoprotein. The IL-17F homodimer includes a classical cysteine knot motif, which is found in the TGF-beta, bone morphogenetic protein, and nerve growth factor superfamilies. One difference in the cysteine knot motif of IL-17F compared with the other known cysteine knot protein families is that it only utilizes four cysteines instead of the classical six cysteines to form the knot. IL-17F and IL-17A have been shown to form biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F and IL-17A homodimers.
Components
Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human IL-17F
Biotin-Conjugate anti-human IL-17F polyclonal antibody
Streptavidin-HRP
Human IL-17F Standard lyophilized, 4 ng/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
京公网安备11010802025653 版权所有：北京逸优科技有限公司
