Description: The human MIP-1alpha Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of MIP-1alpha levels in cell culture supernatants, serum and plasma. The human MIP-1alpha Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.
MIP-1alpha and MIP-1beta belong to the family of cysteine-cysteine (cc) chemokines, RANTES being another prominent member thereof.
Both MIP-1alpha and MIP-1beta are not only chemoattractants but also coactivators of macrophages acting in concert with IFN-gamma as type 1 cytokines.
MIP-1alpha and MIP-1beta are distinct but highly homologous chemokines produced by a variety of host cells in response to various external stimuli and share affinity for their receptor CCR5. The roles of MIP-1alpha and MIP-1beta have been elucidated in response to their affects on cellular and humoral immune response. MIP-1alpha was shown to stimulate strong antigen specific responses, while MIP-1beta promotes antibody responses. Both macrophage inflammatory proteins are however strictly associated with type 1 immune response.
Determination of the expression levels of the MIP-1s turned out to provide important information regarding numerous diseases such as multiple myeloma, allergic asthmatic disorders, acute experimental autoimmune encephalomyelitis, HIV infection, sarcoidosis and sepsis.
Components
Aluminium pouch(es) with a Microwell Plate coated with Polyclonal Antibody to MIP-1alpha, Biotin-Conjugate (anti-MIP-1alpha polyclonal antibody), Streptavidin-HRP, and Sample diluent, lyophilized
Aluminium pouch(es) with a human MIP-1alpha Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Sample Diluent (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers
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