Description: The human IL-23 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-23. The human IL-23 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
This ELISA kit is specifically engineered for accurate and precise measurement of IL-23 protein levels from samples including serum, plasma, and supernatants from cell cultures. The use of a p19-specific capture antibody and a p40-specific detection antibody renders this sandwich ELISA exquisitely specific for human IL-23.
IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked to p19. Like p35 of IL-12, p19 is biologically inactive by itself. IL-23 interacts with IL-12R beta -1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-gamma production by na‹ve and memory T cells, as compared to IL-12.
eBioscience's recombinant IL-23 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p19 and p40 subunits using a dual promoter expression system. It is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker.
onents
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-23
Biotin-Conjugate anti-human IL-23 monoclonal antibody
Avidin-HRP
Human IL-23 Standard, lyophilized, 4000 pg/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
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