Description: The human MMP-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of total human MMP-3. The human MMP-3 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
The matrix metalloproteinases (MMPs) represent a family of more than 15 Zn-dependent endopeptidases with extracellular activity. They share a common conserved protease domain sequence in which three His residues form a complex with a catalytic Zn ion. These enzymes are responsible for the degradation and remodelling of extracellular matrix (ECM) components such as collagen, aggrecan, laminin, fibrilin and fibronectin.
MMP-3 also known as, matrix metalloproteinase-3 or stromelysin-1, is secreted as a zymogen by connective tissue cells that requires protelytic cleavage of the attached pro-peptide domain for activation. Increased serum levels of MMP-3 are observed in inflammatory rheumatic diseases. MMP expression also correlates strongly with invasive behavior of tumor cells in culture and with metastasis in animal models. Overexpression of SL-1 can facilitate cell infiltration into surrounding tissue and degrade the ECM leading to induction of apoptosis.
MMP-3 levels are a good marker for several inflammatory disease states.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human MMP-3
Detection Antibody Mixture containing unlabeled anti-human MMP-3 polyclonal antibody (rabbit) mixed with goat anti-rabbit-HRP
human MMP-3 Standard lyophilized, 8 ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films
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