Description: The human IL-12/IL-23 p40 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-12/IL-23 p40. The human IL-12/IL-23 p40 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Biologically active IL-12 is a disulfide-linked heterodimeric 70-kDa cytokine composed of a 35-kDa (p35) and a 40-kDa (p40) subunit. P35, a member of the IL-6 superfamily, is secreted in response to IFN gamma and agonists of TLR3, 4, or 7. However, p35 expression has been shown to be inhibited by Th2 cytokines and expressed at much lower levels than p40. Expression of p40 is regulated independently of p35. Moreover, p40 has been shown to be secreted as either a monomer or homodimer. Although each subunit alone does not possess IL-12 bioactivity, the p40 homodimer can bind to the IL-12 receptor and act as an antagonist to IL-12 p70. The receptor for this cytokine is composed of two subunits, IL-12R beta 1 and IL-12R beta 2, the latter of which is the signaling component of the receptor.
Components
Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human IL-12/IL-23 p40
Biotin-Conjugate anti-human IL-12/IL-23 p40 monoclonal antibody
Streptavidin-HRP
Human IL-12/IL-23 p40 Standard lyophilized, 4 ng/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films