Description: The human TRAIL ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TRAIL.
TRAIL (TNF-related-apoptosis-inducing-ligand) was found to be a member of the TNF family which mediates cell death in a wide variety of malignant cell lines and primary tumor cells. TRAIL induces two different signals, cell death mediated by caspases and gene induction mediated by NFkappaB.
The cytotoxic ligand TRAIL interacts with five receptors, only two of which (TRAIL-R1 and R2) have a death domain.
TRAIL, which is ubiquitous expressed, exhibits very complex signalling and tumor-selective induction of apoptosis. TRAIL is involved in lymphatic neoplastic disorders and thyroid diseases, and the treatment of cancer like melanoma.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human TRAIL
Biotin-Conjugate anti-human TRAIL monoclonal antibody
Streptavidin-HRP
Human TRAIL Standard lyophilized, 2 ng/ml upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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