Description: The human Iso-Insulin Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Insulin levels in cell culture supernatants, human serum or plasma. The human Iso-Insulin Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Insulin is a key player in the control of carbohydrate metabolism. It facilitates the entry of glucose into muscle, adipose and several oher tissues. Insulin is composed of two chains referred to as A-chain (21 amino acids) and B-chain (30 amino acids) which are connected by two disulfide bonds, with an additional disulfide bond within the A-chain. The Insulin synthesis takes place in the beta-cells of the islets of Langerhans in the pancreas. Insulin secretion is mainly caused by elevated glucose concentration of plasma. Proinsulin is cleaved into Insulin and C-peptide, which are then secreed to circulation in equimolecular amounts.
Very low levels of Insulin are observed with diabetes mellitus type I, and hypopituitarism. Diabetes mellitus type II, obesity, insulinoma, cushing?s syndrome and acromegaly show significantly raised Insulin levels.
Components
Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human Insulin, HRP-Conjugate (anti human Iso-Insulin monoclonal antibody) and Sample Diluent lyophilized
Aluminium pouch(es) with a human Iso-Insulin Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Sample Diluent (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers
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