G Alp q Pure 10/GAQ 150ug

The GTP binding regulatory proteins (G proteins) consist of three subunits: α, β, and γ. These heterotrimeric proteins function at membranes

to relay signals from cell surface receptors to intracellular effectors. The α subunit is unique for each G protein and contains the site of GTP

binding and hydrolysis, as well sites for receptor and effector interactions. The βγ subunit complex interacts directly with receptors and the α

subunit. The Gα family includes four families: the Gαs family including Gαs , Gαo1f, and Gαt, the Gαi family including Gαi, Gαo, and Gαz,

the Gαq/Gα11 family and the Gα12/13 family. The Gαq protein is 88% homologus with Gα11 and both are widely expressed. These G

proteins activate phospholipase C proteins, which induce calcium signaling events. G protein coupled receptors (GPCRs) involved in

regulating Wnt signaling activate Gαq, phospholipase Cβ, and induce calcium-dependent activation of calpain. These events promote

β-catenin nuclear export and proteolysis. Gαq has also been implicated in metabotropic glutamate receptor signaling. Thus, Gαq isoforms

activate phospholipase C proteins in various G-protein coupled receptor pathways.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.