Fyn (pY528)/c-Src (pY530) Pure 31 50ug

p59fyn is a member of the Src family of protein tyrosine kinases. Two isoforms of the protein have been identified, designated as FynB and

Fyn T. Fyn B has been shown to be localized to the brain, whereas Fyn T associates with both B and T cells. Stimulation of the T cell antigen

receptor (TcR) results in protein tyrosine phosphorylation via non-receptor tyrosine kinases. p59fyn kinase (Fyn T) associates with the TcR.

Ligation of the TcR activates the protein kinase activity of p59fyn in various human T cells. Fyn interacts with the CD3-zeta chains through its

N-terminal region. In turn, fyn binds other proteins through its SH2 and SH3 domains. These proteins (p82 and p116) may serve as substrates

and/or mediators of fyn activity. p59fyn kinase is tyrosine phosphorylated at two sites, Tyr-417 (autophosphorylation site), and Tyr-528

(negative regulatory site).

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.