Drosophila Karyopherin Alp2 Pure 2 50ug

The two step process of importing proteins into the nucleus involves the binding and interaction of several cytosolic and nuclear pore proteins.

Proteins to be translocated into the nucleus contain a nuclear localization sequence (NLS) which is recognized and bound by carrier proteins

in the cytosol. Heterodimers belonging to a highly conserved family of proteins called karyopherins are required for successful nuclear

localization of cytosolic proteins. The α-subunits appear to function in the binding of NLS (both simple and bitartite NLS motifs), but both α-

and β-subunits are required for successful docking to the nuclear envelope. ATP is required for complete translocation of proteins into the

nucleus. Karyopherin α2 was first identified as Rch-1, an NLS receptor which interacts with the RAG-1 recombination-activating protein in

developing B and T cells. Rch-1 has been reported to be 44% identical to karyopherin α1 (hSRP-1 /NPI-1).

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.