Sec31A FITC 32/Sec31A 50ug

Eukaryotic protein trafficking involves packaging of target molecules into membranous vesicles that bud from a donor compartment, travel to

a specific destination, fuse, and release their contents into an acceptor compartment. Vesicles that bud from the Golgi cisternae and from the

ER contain a non-clathrin based coat. This coat is an oligomeric complex whose subunits are termed COPs (coatomer proteins). COPI is the

golgi- and endosome-associated COP complex, while COPII is the ER-associated coat complex. In yeast, the COPII complex has a cargo

binding subcomplex that includes Sar1p, Sec23p, and Sec24p, which recruits Sec13p and Sec31p leading to vesicle formation. The human

homologues of Sec31p are Sec31A and Sec31B. Sec31A has 40% identity with Sec31B, and contains an N-terminal WD-40 domain and a

C-terminal proline-rich region. Sec31A mRNA is widely expressed, while Sec31B is found only in testis and thymus. In HeLa, Sec31A

localizes to vesicular structures in the perinuclear region of the cell, and co-localizes with the COPII component Sec13. Antibodies against

Sec31A inhibit ER to Golgi transport of vesicular stomatitis G protein. Thus, Sec31 may be an important COPII component involved in ER to

Golgi transport, as well as a marker for COPII vesicles.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.