MAD2B/Rev7 Pure 14/MAD2B/Rev7 50ug

Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins.

These events are of utmost importance at the checkpoints, or transition points, of the cell cycle. MAD2 (Mitotic Arrest-Deficient) is the human

homolog of a yeast and Xenopus protein that is essential for spindle assembly during mitosis. Binding of affinity purified polyclonal

antibodies to the MAD2 protein has been reported to prevent mitosis of HeLa cells. Furthermore, MAD2 is localized at the kinetochore of

condensed chromosomes during mitosis and cells defective in the mitotic checkpoint have reduced levels of MAD2. MAD2 inhibits

anaphase-promoting complex (APC) ubiquitin ligase activity,which is critical for controlling transitions in mitosis. This inhibition occurs

through binding and suppression of CDC20 activation of APC. MAD2B, also known as Rev7, is a UV revertible gene that has 25% identity

with MAD2 and also acts as an inhibitor of APC. Interestingly, MAD2B/Rev7 can bind MAD2 and interacts with the DNA polymerase ζ

subunit Rev3. Thus, MAD2B may interact with mitotic checkpoint proteins and DNA repair proteins to regulate mitotic progression.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.