DNA topoisomerases are ubiquitous nuclear enzymes that alter the topology of DNA. Topoisomerase I produces transient single-stranded
breaks in DNA, while topoisomerase II produces transient double-stranded breaks in DNA. These DNA breaks are important for DNA
replication and chromosome condensation and segregation. Two types of topoisomerase II have been identified, TopoIIα and TopoIIβ. During
interphase, TopoIIα is found in the nucleoplasm and nucleoli, and in mitosis, TopoIIα is found associated with metaphase chromosomes and
centromeres. Transcriptional control of TopoIIα expression is regulated through multiple gene promoters, which include five inverted CCAAT
boxes, one ATF binding sequence, and two GC-rich boxes. ICBP90 was identified in one-hybrid screen of proteins that can bind an inverted
CCAAT box. ICBP90 is expressed highest in proliferative tissues such as thymus, liver, and bone marrow. Overexpression of ICBP90 in
COS-1-transfected cells leads to enhanced expression of TopoIIα. ICBP90 co-localizes with TopoIIα in proliferating areas of appendix and in
high grade breast carcinomas. Thus, ICBP90 may be an important transcripitonal regulator of TopoIIα expression in proliferating cells.
原厂资料：
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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