TNIK Pure 53/TNIK 50ug

Ste20 is a S. cerevisiae Ser/Thr protein kinase that functions upstream of the MAP kinase module. Mammalian and yeast homologs of this

kinase are divided into two classes based on their structure and regulation. Members of the first class (Ste20, Cla4, and p21-activated protein

kinase) contain a C-terminal kinase domain, an N-terminal regulatory domain and a small GTPase Rac1/Cdc42 binding domain. Members of

the second class lack the GTPase binding sites, but are similar to the former class throughout the catalytic domain. This second class includes

germinal center kinase (GCK), HPK, KHS, KRS1 and 2, MST1, 2, and 3, NIK, SOK-1, and TNIK. Traf2- and NCK-interacting kinase (TNIK)

is most homologous to another NCK-interacting kinase, NIK. TNIK contains an N- terminal kinase domain, and a C-terminal GCK homology

(GCKH) domain. The mRNA of TNIK is expressed highest in heart, brain, and skeletal muscle. Overexpression of TNIK activates the JNK

pathway, and leads to the disruption of F-actin structures and the inhibition of cell spreading. In vitro, TNIK can phosphorylate gelsolin.

Thus, TNIK is a GCK family kinase that may regulate both the JNK pathway and cytoskeletal dynamics.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.