Synaptojanin 1 Pure 26/Synaptojanin 50ug

Phosphoinositide turnover is a mechanism of intracellular signaling that involves phosphorylation of the inositol ring to produce signaling

molecules that include phosphoinositol (PI)-3-P, PI-3,4-P2, PI-3,4,5-P3, PI-4-P, and PI-4,5-P2. These moleclules function in many signaling

pathways to regulate events at cell membranes, such as endocytosis, exocytosis, and cell morphology. Synaptojanin 1 is a phosphoinositide

phosphatase that selectively cleaves the 3-, 4-, and 5- phosphates from PIs. Two splice variants of synaptojanin 1 include a 145 kDa form that

is found in adult brain, and a 170 kDa that is widely expressed in non- neuronal cells. The 145 kDa form contains a Sac1 domain that has

phosphoinositide phosphatase activity, a second phosphatase domain that has only PI-5 phosphatase activity (PI-5), and a proline-rich

SH3-binding domain. The 170 kDa form contains these domains, as well as a second SH3-binding consensus sequence. Synaptojanin binds

many proteins implicated in endocytosis, including amphiphysins and endophilin. Thus, synaptojanin 1 may regulate endocytosis through

modification of the interactions between SH3-domain proteins and PIs.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.