p21-Arc FITC 26 50ug

Cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton. This reorganization of actin filaments

occurs through the interactions between actin and actin binding proteins. Actin-binding proteins regulate the polymerization and

depolymerization of actin, connect actin-based structures to membranes and to other cytoskeletal elements, power the movement of actin

filaments, and cross-link actin filaments into bundles. Actin related proteins (Arp) 2/3 complex is an actin polymerization inducing complex

that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20 Arc, and p16-Arc. The Arp2 and Arp3 subunits may nucleate actin polymerization,

while the p41-Arc subunit is a WD repeat-containing protein that may regulate both the activity and localization of the Arp2/3 complex.

Arp3, p34-Arc, and p21-Arc are localized to the lamellipodia of stationary and locomoting fibroblasts. Both WASP and Abp1p are acidic

sequence-containing proteins that activate the Arp2/3 complex. However, WASP binds actin monomers, while the endocytosis-related Abp1p

protein binds actin filaments. Thus, Arp2/3 complex may regulate actin polymerization in specific cell locations through interaction with actin

binding Arp2/3 activators.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.