Stat1 (pY701) Pure 4a 50ug

Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including

interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK

family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the

nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed

and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated

Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface

receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1α and 84-kDa Stat1β; Stat1α has 38 additional C-terminal amino acids. In

response to the binding of IFNα, IFNγ, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become

tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that

includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes

(ISGs).

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.