PBK Pure 31/PBK 150ug

Mitogen activated protein (MAP) kinase signal transduction pathways mediate the effects of various extracellular stimuli on biological

processes such as proliferation, differentiation, and death. The p38 MAP kinases are activated by dual phosphorylation on Thr and Tyr within

the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the MAP kinase kinases,

MKK3 and MKK6. PBK is a PDZ-binding Ser-Thr kinase related to the MKK3/6 MAPKK family. PBK was also identified as T-LAK celloriginated

protein kinase (TOPK) and spermatogenesis-related protein kinase (SPK). PBK contains the conserved dual specficity active site

sequence (D-X-K-X-X-N) at amino acids 174 to 179, and a C-terminal ETDV motif that binds PDZ domains. The mRNA expression of PBK

is reportedly highest in placenta, and lower in heart and pancreas. In mitotic cells, PBK is phosphorylated and enzymatically active, and in

vitro, PBK is phosphorylated by Cdc2/cyclin B. PBK can bind to the PDZ domain-containing protein, Dlg, and can phosphorylate p38

MAPK. Thus, PBK is a MAPKK family member that may be important for regulation of PDZ domain proteins during mitosis.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.