Regulation of transcription is mediated by transcription factors that bind regulatory elements in gene promotors and enhancers, and alter the rate of transcription. Transcriptional repressors reduce transcription by their intrinsic repressor domain activity, or by binding to sites in the regulatory domain where a co-repressor can bind. GC-binding factors (GCF and GCF2) are transcriptional repressors that bind promoters with GC-rich sequences. GCF represses transcription of EGF, TGFα, and insulin-like growth factor II recepetors. GCF2 has some homology with GCF, and has also been identified as TRIP and LRRFIP1. The sequence of GCF2 includes an RNA binding domain (RBD), and N-glycosylation site, and a nuclear localization signal (NLS). GCF2 is widely expressed in many tissues and cells. GCF2 binds the EGFR and PDGFR promoters, and represses both EGFR and PDGFR transcription. In addition, GCF2 interacts with the leucine-rich repeat of flightless I, a protein that binds actin and contains a gelsolin-like domain. Thus, GCF2 may have functions in both RNA-cytoskeleton interactions, as well as roles in transcriptional repression.
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1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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