Transferrin Receptor Pure 2 150ug

Iron acquisition is a prerequisite for cell proliferation. The transferrin receptor is the primary mode of iron uptake. This receptor mediates iron

uptake through internalization and recycling of the iron carrying serum protein transferrin. Binding of transferrin to its receptor causes rapid

internalization into an acidic intracellular compartment where iron dissociates from transferrin. Both the receptor and transferrin are then

recycled through the exocytic pathway. The transferrin receptor is expressed in most cells and tissues, especially proliferating cells, and the

receptor is expressed as a dimer of identical intergral membrane proteins. Transferrin receptor mRNA expression is inversely proportional to

iron availability, and this regulation occurs through post-transcriptional control of mRNA stability. Transferrin receptor mRNA contains iron

responsive elements (IRE) that are bound by IRE-binding proteins (IRP). The RNA binding of IRPs is dependent on iron levels. Thus, iron

deprivation leads to increased RNA binding, which masks the recognition site of endonucleases and stabilizes transferrin receptor mRNA.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.