Beclin Pure 20/Beclin 150ug

Autophagy is the bulk degradation of cellular proteins through the autophagosomic-lysosomal pathway. This process is important for normal

cell growth, and may be disrupted in tumor cells. Beclin is a Bcl-2 binding protein that is homologous to the yeast autophagy gene,

apg6/vps30. The structure of beclin includes a Bcl-2 binding coiled-coil region, and a leucine-rich nuclear export signal (NES). Beclin protein

colocalizes with intracytoplasmic organelles and nuclei in normal COS7 and MCF7 cells. However, inhibition of CRM1-dependent nuclear

export leads to beclin localization primarily in the nucleus. Mutation of the NES domain of beclin also prevents nuclear export, as well as

suppresses beclin-mediated nutrient deprivation-induced autophagy. In addition, these beclin mutants can not inhibit in vitro clonigenicity and

in vivo tumorigenicity of MCF7 cells. Beclin interaction with Bcl-2 may be involved with host viral defense, since overexpression of beclin

inhibits Sindbis virus replication and expression of beclin lacking the Bcl-2 binding domain has no antiviral effects. Thus, beclin may be an

important component of complexes involved in autophagy.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.