SGT1 Pure 29/SGT1 50ug

Maintenance of cellular function requires timely and selective degradation of key regulatory proteins. For example, progression of the

mammalian cell cycle is regulated by the degradation of key cell cycle proteins via the ubiquitin pathway. Ubiquitin, a soluble protein of 76

amino acids, is enzymatically attached to an ε-NH2-Lys in a target protein. Ubiquitin-conjugated proteins are recognized and degraded by the

26S proteasome. Targeting of specific cellular proteins for degradation via the ubiquitylation pathway may require the formation of protein

complexes. One group of ubiquitin enzyme containing complexes are the multisubunit cullin-containing RING E3 ubiquitin ligases, that

include SCF and VCB-Cul-2 complexes. These complexes contain a RING domain protein, a cullin, multiple adaptor proteins, and ubiquitin

enzymes. The SCF complex includes Skp1, SGT1, Cul-1, F-box protein, and CDC34/Ubc3. This complex preferentially ubiquitylates

phosphoproteins, such as β-catenin, IκBα, and G1 cyclins. The mammalian SGT1 is an adaptor protein that can interact with Skp1 and can

rescue yeast SGT1 null mutations. Thus, SGT1 may be an important component of the SCF ubiquination ligase complex.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.