NIH 3T3/Vanadate/PDGF Lysate 500ug

产品编号：BD-612069 品牌：BD-Pharmingen 原厂货号：612069
产品分类：蛋白质表达与纯化 > 蛋白质样本制备 > 细胞组织粗提物与裂解物
应用分类：

包装：

500ug

运保温度:

Store undiluted at -20° C.

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描述：

Cell or tissue lysates are a useful control for the western blotting application. A source that can be rich in endogenously expressed proteins, this lysate is convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel. This lysate was routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

注意事项：

Store undiluted at -20° C. Do not dilute or boil. NIH 3T3 cells were serum starved overnight and then incubated at room temperature for 10 minutes in a final concentration of 1 mM sodium vanadate. PDGF was then added at a final concentration of 5 ng/ml and cells were incubated at 37°C for 30 minutes. Western blots of NIH 3T3 cell lysates treated with vanadate and PDGF have been reported to show a shift in SHC mobility in addition to phosphorylation of PKBα/Akt and Stat3 as compared to untreated control cells.