eIF-5a Pure 26/eIF-5a 50ug

Initiation of eukaryotic translation involves a series of reactions mediated by multiple eukaryotic initiation factors (eIFs). However, one

member of this family, eIF-5a, may have multiple protein functions. eIF-5a was originally identified as an initiation factor due to its binding of

ribosomes, and its stimulation of methionyl-puromycin. Regulation of initiation may not be the major function of eIF-5a, since it functions as

a targeting protein for the HIV protein, Rev, and the T-cell leukemia virus type 1 protein, Rex. In Xenopus oocytes, eIF-5a interacts with the

nucleoporins Nup214, Nup153, Nup98, and Nup62, and is required for Rev-NES interaction with exportin1. These interactions may be

involved with eIF-5a nuclear export of Rev protein, and eIF-5a requirement for Rev-mediated viral RNA export. eIF-5a is ubiquitously

expressed, and is the only known eukaryotic protein that contains the post-translationally formed hypusine residue. Modification of eIF-5a

with hypusine is coupled to cell proliferation, and disruption of this residue in yeast eIF-5a is lethal. Thus, eIF-5a may be an important nuclear

transport protein with conserved function in many species.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

3.This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.