Stat5 (pY694) Pure 47 50ug

The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Seven mammalian STATs have been

identified: Stat 1-4, Stat5a, 5b, and Stat6. The SH3 domain of Stat5 shows extensive homology with the SH2 domains of Stat1 and Stat2. This

homology leads to speculation that it may have a broader physiological role than originally anticipated and has led to its classification as a

member of the Stat family. Stat5 has been further characterized and shown to be encoded by two separate genes, Stat5a and Stat5b that share

over 90% identity at the amino acid level. Further insight into the exact biological role of Stat5a and Stat5b was determined with the

generation of Stat5a and Stat5b deficient mice. Stat5a was shown to be involved in lactogenesis and mammary development, while Stat5b was

shown to be involved in growth hormone signaling and to play a role in liver gene expression. Both Stat5a and Stat5b share similarities, both

are involved in IL-2 induced peripheral T cell proliferation. Stat5 (MGF) mRNA expression is highest in mammary gland tissue. Lower levels

are found in ovary, thymus, spleen, kidney, lung, muscle, and the adrenal gland. The peptide hormone, prolactin, binds to the prolactin

receptor (PRLR) to initiate the lactogenic response. There are at least three forms of PRLR; however, only the long form is able to activate the

92 kDa Stat5 protein by inducing phosphorylation at Tyr694. Once phosphorylated, Stat5 becomes an essential transcription factor which

binds to the β-casein gene promoter. Stat5 activity is tightly regulated throughout gestation, lactation, and post-lactation. Treatment of

activated Stat5 with a protein tyrosine phosphatase results in the loss of DNA binding activity. The presence of an SH2 domain within Stat5

suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.