Synaptophysin is a major integral membrane glycoprotein that was isolated from the presynaptic vesicles of neurons. The structure of
synaptophysin includes four transmembrane domains (TMD), an N-temrinal glycosylation site, nine repeats of basic tetrapeptide motifs
(GYGP), multiple collagenase sensitive sites (XGP), and several collagen-type tripeptides (GXX). Synaptophysin mRNA is expressed in
nervous tissues, as well as many neuroendocrine tumor cell lines. In PC12 cells and brain synaptic vesicles, synaptophysin binds cholesterol,
and cholesterol depletion blocks the biogenesis of synaptic-like microvesicles. In addition, synaptophysin binds dynamin in a Ca2+-dependent
manner, and injection of peptides that disrupt this interaction decreases neurotransmitter release during high-frequency stimulation of squid
neurons. This decrease in neurotransmitter release results from inhibition of synaptophysin-dependent vesicle retrieval. Thus, synaptophysin
may have important roles in the biogenesis and recycling of synaptic vesicles. Synaptophysin is an integral membrane glycoprotein with a
calculated molecular weight of 33 kD, but has been reported to be observed at 38-42 kD due to glycosylation.
原厂资料：
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
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