Normal epithelial cell-specific 1 (NES1) was identified in a screen of genes whose mRNA is down-regulated during oncogenic transformation of human mammary epithelial cells (MECs). The protein is homologous to multiple serine proteases including members of the trypsin and kallikrein protease families. Similar to other serine proteases, the sequence of NES1 contains three residues (Ser229, Asp137, and His86) that form a catalytic triad and a putative cleavage site (Arg42). The pattern of NES1 expression in normal human tissues includes prostate, testis, ovary, small intestine, colon, and lung. In addition, expression has been reported to be reduced in human breast and prostate cancer cell lines. NES1 is a secreted protein found in the supernantant of growing MECs. Expression of NES1 in a breast cancer cell line has been reported to suppress oncogenic cell behavior, such as anchorage-independent growth and tumor formation in nude mice. In the breast cancer cell line BT-474, NES1 levels reportedly are up-regulated by steroid hormones, such as estrogen, androgen, and progestin. Thus, NES1 is a steroid-hormone regulated, serine protease that is thought to have important tumor suppressor functions.
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注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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