hCNK1 Pure 46/hCNK1 50ug

Proteins of the Ras superfamily play critical roles in the control of normal and neoplastic proliferation. These proteins relay signals from

Tyr-kinases at the plasma membrane to the nucleus via a network of Ser/Thr kinases that includes the MAP kinase (Raf-MEK-ERK) pathway.

Kinase suppressor of Ras (KSR-1) was discovered in Drosophila in a genetic screen that identified mutations that suppress constitutively

active Ras mutants. Connector enhancer of KSR (CNK) was found in a similar screen to identify mutations that enhance the KSR- dependent

phenotype. Drosophila CNK contains a sterile alpha motif (SAM), a conserved region in CNK (CRIC), and a PDZ domain in the N-terminal

region, a proline-rich and a plecstrin homology (PH) domain in the central region, and a C-terminal Pro-rich domain. The human homologue

of CNK (hCNK1) contains similar N-terminal and central domains, but is 713 amino acids in length compared to 1557 amino acids for

Drosophila CNK. In Drosophila, the N-terminal region of CNK facilitates binding to RAS, while the C-terminal region inhibits RAS- and

RAF-dependent signaling. Thus, CNK may be important for regulation of both RAS- and RAF-dependent signaling.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.