LITAF Pure 30/LITAF 50ug

TNFα (Tumor Necrosis Factor α) is a pleiotropic cytokine that conveys either beneficial (prevention of cancers and infection, activation of

inflammatory responses) or detrimental (mediation of septic shock, inflammation, and apoptosis) effects to the host. It is produced and

secreted primarily by monocytes and macrophages in response to stimulation with lipopolysaccharide (LPS), a principle endotoxic component.

LPS-induced TNFα factor (LITAF) is a nuclear factor that was purified as a result of its ability to bind to a DNA fragment from the TNFα

promoter region. LITAF expression is dependent on LPS induction and PMA differentiation in THP-1 cells and is observed at high levels in

spleen, lymph node, and peripheral blood leukocytes. Antisense inhibition of LITAF causes a reduction in TNFα expression in THP-1 cells. In

addition, p53 induces the expression of a transcript called PIG7, which has 98% homology with LITAF. Thus, LITAF is thought to link p53

pathways with the induction of TNFα gene expression in response to LPS stimulation and to function, possibly as a transcription factor, to

regulate the expression of this highly potent cytokine. This antibody was generated to the LITAF aa 1-212 sequence (Genbank accession #

U77396). Recent reports indicate that the nucleotide coding sequence for LITAF may differ and instead, encode for a protein designated as

SIMPLE. Under the sequence for SIMPLE, the LITAF aa 1-212 sequence used to generate this antibody corresponds to the aa 1-126 region of

SIMPLE.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.