TAP Pure 31/TAP 50ug

Nuclear export of cellular mRNAs is highly selective, wherein only fully processed RNAs are exported. Retroviral replication requires export

of unspliced viral RNAs, which serve as templates for structural proteins and as genomic RNA for progeny virus. The export of unspliced viral

RNA depends on the interaction of cis-acting RNA elements with cellular factors. In simian type D retroviruses, the cis-acting element is the

constitutive transport element (CTE). CTE-dependent nuclear transport is mediated by the cellular TAP protein. TAP (Tip associating protein)

is the vertebrate homolog of the yeast nuclear export protein Mex67p. TAP is a nuclear protein that shuttles between the nucleus and

cytoplasm. It contains an N-terminal nuclear localization and export region (NLS-NES), a central RNA binding domain (RBD), and a

C-terminal portion that is required for nuclear localization and nuclear rim association. The first 372 aa of TAP are necessary and sufficient for

binding and nuclear export of CTE-containing RNA. Thus, TAP contains a novel RNA-binding motif that recognizes CTE-containing RNA

and interacts with other components of the nuclear transport machinery.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.