MnSOD Pure 19/MnSOD 50ug

Superoxide dismutase (SOD), catalase, and glutathione peroxidase are necessary for preventing the intracellular accumulation of reactive

oxygen species. Three forms of SOD include the cytosolic Cu/Zn2+ SOD, the extracelluar Cu/Zn[2+] SOD, and the mitochondrial manganese

(Mn) superoxide dismutase (MnSOD). In the mitochondria, MnSOD catalyzes the dismutation of two superoxide radicals, producing H2O2

and oxygen. Following protein synthesis, MnSOD is post-transcriptionally modified for transport from the cytosol into the mitochondria. In

rat hepatocytes, H2O2 exposure induces MnSOD expression, while in rat lung, exposure to smoke induces expression in bronchial epithelial

cells. In addition, MnSOD expression is required for PC12 cell resistance to nitric oxide (NO) toxicity and for nNOS neuron resistance to

NMDA-induced NO toxicity. Superoxide radical anions may induce transcriptional activation of the MnSOD gene through PKC

phosphorylation and activation of a CREB-1/ATF-1-like factor. Thus, MnSOD removal of reactive oxygen species created by exposure to

toxic stimuli is thought to be critical for the survival of a variety of cell types.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.