NCS-1 Pure 1/NCS-1 50ug

Ca2+-signaling pathways are critical for a variety of cell processes, such as gene expression, cytoskeletal dynamics, cell cycle, cell death,

synaptic transmission, and basic cellular metabolism. A variety of EF-hand containing Ca2+-binding protein families have been implicated in

neuronal Ca2+-regulated events. For instance, the neuronal Ca2+ sensor family (recoverin, neurocalcin, hippocalcin, VILIP, and neuronal Ca

2+ sensor-1 (NCS-1) have been implicated in phototransduction signaling in photoreceptor cells and in exocytosis in presynaptic terminals.

NCS-1 is the mammalian homologue of the Drosophila Ca2+-binding protein, frequenin. It contains four EF-hand motifs and an N-terminal

myristolation site and is expressed in neurons, chromaffin cells, and glial cells. Overexpression of NCS-1 in chromaffin and PC12 cells

increases Ca 2+-regulated exocytosis of dense-core granules. In vitro, NCS-1 activates a variety of enzymes, including calcineurin, cyclic

nucleotide phosphodiesterase, and nitric-oxide synthase. Thus, NCS-1 is a Ca2+-binding protein that regulates a variety of cell signaling

pathways involved with neuronal exocytosis.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.