KSR-1 Pure 15/KSR-1 50ug

Proteins of the Ras superfamily play critical roles in the control of normal and neoplastic proliferation. In mammalian cells there are four true

Ras proteins (encoded by Ha-ras, N-ras, Ki-rasA, and Ki-rasB). These proteins relay signals from tyrosine kinases at the plasma membrane to

the nucleus via a network of Ser/Thr kinases that includes the MAP kinase (Raf-MEK-ERK) pathway. Kinase suppressor of Ras (KSR-1) was

discovered in a genetic screen to identify mutations that suppress constitutively active Ras mutants. KSR-1 contains five domains that are

conserved areas (CA) in Drosophila, mouse, and human. CA1 is a unique domain, CA2 is a proline-rich domain, CA3 is a cysteine-rich

domain, CA4 is a Ser/Thr rich region, and CA5 contains a C-terminal kinase domain similar to that of Raf. In COS7 cells, ceramide induces

the autophosphorylation of KSR-1 and KSR-1-induced phosphorylation and activation of Raf. In addition, overexpression of KSR-1 in 293T

cells leads to recruitment of MEK to a 700 kDa protein complex and translocates MEK from the soluble to the membrane-associated fraction.

Thus, KSR-1 may have important kinase and scaffolding activities during Ras-related signaling.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.