Phospholipase C Bta 4 Pure 56 50ug

Phospholipase C (PLC) hydrolyzes inositol phospholipids into diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Multiple distinct PLC

isoenzymes have been identified and divided into three structural types: α, β, and γ. This classification is based primarily on the location of the

conserved X and Y domains, whose structural integrity is essential for a functional catalytic core. The activation of PLCβ isoenzymes is

uniquely regulated by G protein subunits, while PLCγ is activated following phosphorylation by protein tyrosine kinases. The β subfamily of

PLC consists of at least four members: β1, β2, β3, and β4. PLCβ4 differs from the other members in that it is not activated by G protein βγ

subunits, it is not found in the liver or kidney, and it is inhibited by ribonucleotides. Various isoforms of PLβC4 result from alternative

splicing or proteolytic cleavage. PLCβ4 is expressed in retina and brain and knockout mice display ataxia and abnormalities in metabotropic

glutamate receptor function in the cerebellum. Thus, PLCβ4 is primarily found in neuronal tissues where it is thought to be important in

neurotransmitter signaling pathways.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.