DBP2 Pure 24/DBP2 50ug

Splicing, the removal of introns from pre-mRNA, is mediated by spliceosomal complexes and occurs in two distinct catalytic steps. The first

step involves cleavage of the 5' exon and the production of a lariat intermediate. In the second step, the 3'-splice site is cleaved and the exons

are fused with the concomitant release of the intron lariat. The splicing process involves several conformational rearrangements of the

spliceosome that are catalyzed by members of the DEAD/H-box superfamily of RNA helicases. Helicases induce unwinding of

double-stranded DNA and RNA in metabolic processes. In S. cerevisiae, helicase proteins that contain the typical superfamily II DEAD/H-box

are splicing factors. DBP1 and DBP2 (DEAH-box protein) are putative human helicases and members of the DEAH-box helicase family.

DBP2 is expressed in a wide range of human tissues with highest expression in heart, skeletal muscle, and testis. In HeLa cells, DBP2

localizes to the nucleus. In addition, DBP2 is a functional homolog of the S. pombe Cdc28/Prp8 protein which is critical for mitosis. Thus,

DBP2 is a putative RNA helicase that is thought to be important in pre-mRNA splicing and cell cycle progression.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.