Stat6 Pure 23/Stat6 150ug

STATs (signal transducers and activators of transcription) are critical mediators of the biologic activity of cytokines including Interleukins (IL)

2-5, IL-7, IL-15, GM-CSF, erythropoietin and growth hormone. Ligand-receptor interaction leads to activation of constitutively associated

JAK family kinases and subsequent recruitment/activation of STATs by tyrosine phosphorylation. Active STATs then move to the nucleus to

promote transcription of cytokine-inducible genes. Seven STAT proteins have been cloned, each of which is differentially expressed and/or

activated in a cytokine-specific and cell type-specific manner. Stat6 plays an important role in signaling pathways that lead to the

differentiation of T helper type 2 (Th2) cells from uncommitted CD4 T cell precursors. Moreover, IL-4, secreted by activated T lymphocytes,

basophils, and mast cells, induces specific gene expression via the induction of tyrosine phosphorylation of Stat6 at tyrosine 641 (Y641). The

SH3:SH2 domain of Stat6 associates with tyrosine-phosphorylated IL-4 receptor and the proximal Jak kinase phosphorylates Stat6 at Y641 on

the C-terminal side of the SH2 domain. Stat6 is then released from the receptor, dimerizes, and is thought to contact the basal transcription

machinery by binding to p300/CBP. While Stat6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood

lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.