p70[S6k] Pure 16/p70[S6k] 150ug

Increased protein synthesis, an obligatory step in cell proliferation, is regulated by the phosphorylation of a number of translational

components, such as ribosomal protein S6. p70 S6 Kinase (p70 [S6K]), a cytosolic kinase that is primarily responsible for S6

phosphorylation, belongs to a family of at least six S6 kinases. p70 [S6K] and p85 [S6K] are two isoforms of the same kinase. Although they

are encoded by the same gene, p80 [S6K] contains a 23 amino acid N-terminal extension that serves as a nuclear localization sequence. These

kinases are located in a p21 ras-independent signaling pathway and are regulated by Ser/Thr phosphorylation in response to mitogenic

stimulation. Activation of p70 [S6K] requires sequential phosphorylations within the autoinhibitory domain and at Thr 389. p70 [S6K]

associates with PDK-1 and PKCζ to form a possible PI3-K signaling complex. The immunosuppressive agent rapamycin forms an

intracellular complex with FKBP12 and FRAP (RAFT1) that inhibits phosphorylation of p70 [S6K] at Thr 389 and, in turn, prevents enzyme

activation. Thus, p70 [S6K] is an important regulator of cell proliferation and a potential target of agents that modify the immune and

proliferative responses. p70 [S6K] has been reported to be observable migrating within a range of 60-70 kD by western blotting.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.