Cell cycle progression is regulated by multiple genes at the G1 to S phase transition. The yeast GST1 gene encodes a GTP-binding protein that can rescue the G1 to S phase transition. This function is conserved in the GST1 human homolog, GSPT1. GSPT1 is also thought to function in protein synthesis as a polypeptide chain releasing factor, eRF3. eRF3 facilitates ribosomal binding of eRF1, a factor which induces ribosomal release of the nascent peptide. In addition to a GSPT1 homolog, a second GSPT protein (GSPT2) has been identified in mice. These proteins have a unique N-terminal region, four conserved GTP- binding domains, and a C-terminal domain with homology to elongation factor-1α(EF1α). Although both GSPTs are expressed in a wide range of tissues, GSPT2 levels are elevated in the brain. GSPT1 expression transiently increases during the G1 to S phase transition, while GSPT2 levels remain constant during all phases of the cell cycle. Both proteins can bind eRF1 and may therefore function as eRF3. Thus, the GSPT proteins play important roles in cell cycle regulation and translation termination and may serve to link these essential biological processes.
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注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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