Enolases are glycolytic enzymes that interconvert 2-phosphoglycerate to phosphoenolpyruvate. They exist as three structurally related but genetically distinct isoforms. The active form of the enzyme is a homodimer formed from one of three subunits (α, β, and γ) that are encoded by distinct genes. The expression of each enolase gene is regulated in a tissue- and developmental- specific manner. While α-enolase has been reported to be expressed in nearly all embryonic and adult tissues, a developmental switch occurs between α and γ isoforms in cells of neuronal origin and between α- and β-enolase in developing skeletal muscle and heart. Early in embryogenesis, β-enolase reportedly is expressed at low levels in skeletal primary fibers. Increases in the expression of β-enolase are detected at the fetal stage of development and after birth. Levels of β-enolase are further increased in adult fast-twitch fibers and with terminal differentiation. In addition, β-enolase expression is regulated during hypoxia via the modulation of Sp1/Sp3 transcription factors levels. Thus, β-enolase is a muscle specific enolase that is thought to be essential for proper development and differentiation of myocytes.
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注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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