GS28 Pure 1/GS28 150ug

Most vesicular fusion events require the function of N-ethylmaleimide-sensitive factor (NSF) and the soluble NSF attachment proteins

(SNAPs). Their function is dependent upon the SNAP receptor (SNARE) family of proteins. The specificity of vesicle docking and fusion is

mediated by specific interactions between v-SNAREs on vesicles and t-SNAREs on target membranes. GS28 (Golgi SNARE with a size of 28

kDa) is a SNARE that associates with the cis-Golgi and participates in trafficking between the ER and the Golgi and between Golgi

compartments. The majority of GS28, the first 230 and 250 aa, is thought to be anchored to the membrane via the C-terminal hydrophobic tail,

which is formed by the remaining 20 aa. GS28 and syntaxin 5, another SNARE, exist as a protein complex in the Golgi and this complex is

important for the function of both proteins in ER-Golgi transport. The GS28/syntaxin 5 complex can be dissociated by α-SNAP and NSF. In

addition, GS28 is thought to interact with α-SNAP when the GS28/syntaxin 5 complex is dissociated. Thus, GS28 is a SNARE protein that

mediates, in complex with syntaxin 5, transport within the Golgi and between Golgi and ER.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.