Initiation of eukaryotic translation involves a series of reactions mediated by multiple eukaryotic initiation factors (eIFs). As a prerequisite to initiation, the initiator methionyl-tRNA (Met-tRNAf), in a ternary complex with eIF-2 and GTP, binds to the 40S ribosomal subunit. This 40S preinitiation complex is positioned at the initiation AUG codon to form the 40S initiation complex. This complex is joined by the 60S ribosomal subunit to form the 80S initiation complex, which functions as a peptidyl transferase. Once translation is completed, the polysomal complex is dismantled with the formation of the free 40S and 60S subunits. eIF-6 is a monomeric protein which binds to the 60S ribosomal subunit and blocks its association with the 40S subunit. The human eIF-6 is expressed in a wide range of tissues and exhibits a significant homology with sequences from S. cerevisiae and Drosophila. The joining of the 60S subunit to the 40S initiation complex requires eIF-5; thus, eIF-5 & -6 may maintain equilibrium between the individual subunits and the 80S complex.
原厂资料：
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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