GOK/Stim1 Pure 44/GOK 50ug

The human chromosomal region 11p15 has undergone intense analysis because of its association with various malignancies. In particular, the

band 11p15.5 contains genes associated with Wilms tumor, Beckwith-Weidemann syndrome, rhabdomyosarcoma, adrenocortical carcinoma,

and lung, ovarian, and breast cancer. One such gene, GOK (Stim 1), was identified near the 5' end of the ribonucleotide reductase subunit 1

gene. Examination of the GOK primary amino acid sequence indicates that it is a typical transmembrane protein with an extracellular

N-terminal domain and a cytosolic C-terminal domain. The protein is highly hydrophobic with only a short region of hydrophobicity that

likely represents the transmembrane region. The C-terminal portion of GOK shares some small regions of homology with myosin (20%

identity). This region of GOK consists of α-helices and is thought to adopt a coiled-coil conformation. Although GOK expression has no effect

on the growth of certain breast cancer cell lines, it induces death in rhabdomyosarcoma cells. Thus, it is thought to be a recessive tumor

suppressor in muscle cells, possibly by functioning as a receptor connected to an apoptotic signaling pathway.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.