PDI Pure 34/PDI 150ug

The ER is the site of translation of membrane and secretory proteins. Following synthesis, it shuttles these proteins through a contiguous

membrane system to their appropriate destinations. Protein disulfide isomerase (PDI) is an abundant, multifunctional, eukaryotic protein.

Although it exhibits ubiquitous expression, it is primarily located in the ER lumen. Here, it functions to catalyze the isomerization of

intramolecular disulfide bridges, thereby allowing them to generate their most thermodynamically stable configurations. This role in

rearrangement has lead to the classification of PDI as a chaperone. Although protein folding occurs in its absence, PDI may be essential for it

to proceed at a physiological relevant rate. In addition, PDI is the β-subunit of prolyl 4- hydroxylase and is a component of the triglyceride

transfer complex. PDI is retained in the ER lumen via its C-terminal -KDEL sequence. Via this sequence, it is continuously recycled back to

the ER from other membranous compartments. Thus, PDI is a diverse protein whose primary function may be to correct disulfide bonding and,

thus, ensure the most stable conformation of newly synthesized proteins.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.