FYB Pure 5/FYB 150ug

Engagement of the T cell receptor (TcR) results in the activation of multiple intracellular src protein tyrosine kinases (PTKs) including p56

[lck] and p59 [fyn]. PTK activation induces TcRζ phosphorylation and the subsequent recruitment and activation of ZAP-70 which

specifically phosphorylates SLP-76. SLP-76 interacts with multiple components of T cell signaling pathways, including Grb-2, PLC-γ, Vav,

and SLAP-130 (also known as SLP-76 Associated Phosphoprotein of 130 kDa). SLAP-130 contains multiple tyrosine based motifs, proline

base type I and II SH3 domain binding motifs, lysine/glutamic acid rich nuclear localization motifs, and an SH3-like domain. It associates with

the SH2 domain of SLP-76 and is phosphorylated by TcR induced PTKs. This protein has also been reported to directly interact with fyn and

has been referred to as fyn binding protein (FYB). Co-transfection data has shown that a limited concentration of FYB/SLAP-130 enhances

TcR-induced IL-2 production, while overexpression downregulates gene expression. Thus, FYB/SLAP-130 is viewed to be a regulatory

component of the fyn/SLP-76 signaling cascade in T cells.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.