CDC42 Pure 44/CDC42 50ug

Rho family members are small GTP binding proteins that serve as molecular switches for a number of biological processes. They cycle

between active GTP-bound and inactive GDP-bound states. CDC42 is a Rho family protein that was identified in membranes of human

platelets and placenta. It is the homologue of CDC42Sc, which regulates initiation of bud-site assembly in Saccharomyces cerevisiae. CDC42

regulates the function of the mammalian actin cytoskeleton, allowing for efficient cytokinesis and cell morphogenesis. CDC42 and Rac1, a

Ras-related GTPase, activate MEKK1, a JNK kinase kinase, which leads to the activation of several downstream components of the MAP

kinase cascade leading to activation of PAK65, a CDC42- and Rac-binding protein. PAK65 interacts with CDC42/Rac1, mediates their

interaction with MEKK1, and enhances MEKK1 catalytic activity. Chronic activation of CDC42 has been shown to induce malignant cellular

transformation. Due to sequence homology among various small GTPase family members, potential cross-reactivity could be observed with

this antibody. BD Pharmingen has not performed such cross-reactivity testing.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.